2981 metabolic genes Search Results


2981 metabolic genes  (Addgene inc)
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Addgene inc 2981 metabolic genes
( A ) Schematic illustration <t>of</t> <t>CRISPR-Cas9</t> screen workflow. ( B ) Top 10 negatively selected genes ranked by robust rank aggregation (RRA) score in 10 Gy–irradiated group compared to the control. ( C ) Manhattan plot of the entire <t>2981</t> metabolic genes by log 10 P value. The top 10 negatively selected genes are highlighted. ( D ) Schematic illustration of the function of LIPT1, LIAS, DLD, and PDHX, the four top hits involved in lipoylation. ( E ) Pathway analysis of the 10 most significantly depleted metabolic pathways by Gene Ontology classification in 10 Gy–irradiated cells compared to the nonirradiated cells. ( F ) Immunoblots of total and lipoylated PDH and α-KGDH subunits (DLAT and DLST, respectively). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), DLAT, and DLST were blotted as loading controls. ( G ) Clonogenic assay of indicated cell lines after 2, 4, 6, and 8 Gy IR. The surviving fraction was normalized to the corresponding sham control, and survival curves were fitted using the linear-quadratic model.
2981 Metabolic Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Schematic illustration of CRISPR-Cas9 screen workflow. ( B ) Top 10 negatively selected genes ranked by robust rank aggregation (RRA) score in 10 Gy–irradiated group compared to the control. ( C ) Manhattan plot of the entire 2981 metabolic genes by log 10 P value. The top 10 negatively selected genes are highlighted. ( D ) Schematic illustration of the function of LIPT1, LIAS, DLD, and PDHX, the four top hits involved in lipoylation. ( E ) Pathway analysis of the 10 most significantly depleted metabolic pathways by Gene Ontology classification in 10 Gy–irradiated cells compared to the nonirradiated cells. ( F ) Immunoblots of total and lipoylated PDH and α-KGDH subunits (DLAT and DLST, respectively). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), DLAT, and DLST were blotted as loading controls. ( G ) Clonogenic assay of indicated cell lines after 2, 4, 6, and 8 Gy IR. The surviving fraction was normalized to the corresponding sham control, and survival curves were fitted using the linear-quadratic model.

Journal: Science Advances

Article Title: Lipoylation inhibition enhances radiation control of lung cancer by suppressing homologous recombination DNA damage repair

doi: 10.1126/sciadv.adt1241

Figure Lengend Snippet: ( A ) Schematic illustration of CRISPR-Cas9 screen workflow. ( B ) Top 10 negatively selected genes ranked by robust rank aggregation (RRA) score in 10 Gy–irradiated group compared to the control. ( C ) Manhattan plot of the entire 2981 metabolic genes by log 10 P value. The top 10 negatively selected genes are highlighted. ( D ) Schematic illustration of the function of LIPT1, LIAS, DLD, and PDHX, the four top hits involved in lipoylation. ( E ) Pathway analysis of the 10 most significantly depleted metabolic pathways by Gene Ontology classification in 10 Gy–irradiated cells compared to the nonirradiated cells. ( F ) Immunoblots of total and lipoylated PDH and α-KGDH subunits (DLAT and DLST, respectively). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), DLAT, and DLST were blotted as loading controls. ( G ) Clonogenic assay of indicated cell lines after 2, 4, 6, and 8 Gy IR. The surviving fraction was normalized to the corresponding sham control, and survival curves were fitted using the linear-quadratic model.

Article Snippet: The human CRISPR knockout pooled library was designed to target 2981 metabolic genes (Addgene, #110066) ( ) and synthesized as a pool of lentiviral vectors containing unique gRNA sequences ( ).

Techniques: CRISPR, Irradiation, Control, Western Blot, Clonogenic Assay